H2N2V1, Main, Exploration, bibRecord, 000176

Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines

Identifieur interne : 000176 ( Main/Exploration ); précédent : 000175; suivant : 000177

Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines

Auteurs : Svetlana Shcherbik [États-Unis] ; Paul Carney [États-Unis] ; Nicholas Pearce [États-Unis] ; James Stevens [États-Unis] ; Vivien G. Dugan [États-Unis] ; David E. Wentworth [États-Unis] ; Tatiana Bousse [États-Unis]

Source :

Virology [ 0042-6822 ] ; 2018.

RBID : PMC:6498426

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Monoclonal (immunology), Antibodies, Monoclonal (isolation & purification), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Cell Line, Cold Temperature, Humans, Influenza A Virus, H2N2 Subtype (growth & development), Influenza A Virus, H2N2 Subtype (immunology), Influenza A Virus, H2N2 Subtype (isolation & purification), Influenza Vaccines (immunology), Influenza Vaccines (isolation & purification), Neuraminidase (immunology), Reassortant Viruses (growth & development), Reassortant Viruses (immunology), Reassortant Viruses (isolation & purification), Technology, Pharmaceutical (methods), Vaccines, Attenuated (immunology), Vaccines, Attenuated (isolation & purification), Viral Proteins (immunology), Virus Cultivation (methods).
MESH :
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Influenza Vaccines, Neuraminidase, Vaccines, Attenuated, Viral Proteins.
- chemical , isolation & purification : Antibodies, Monoclonal, Antibodies, Viral, Influenza Vaccines, Vaccines, Attenuated.
- growth & development : Influenza A Virus, H2N2 Subtype, Reassortant Viruses.
- immunology : Influenza A Virus, H2N2 Subtype, Reassortant Viruses.
- isolation & purification : Influenza A Virus, H2N2 Subtype, Reassortant Viruses.
- methods : Technology, Pharmaceutical, Virus Cultivation.
- Animals, Cell Line, Cold Temperature, Humans.

Abstract

Cold adapted influenza virus A/Leningrad/134/17/57 (H2N2) is a reliable master donor virus (Len/17-MDV) for preparing live attenuated influenza vaccines (LAIV). LAIVs are 6:2 reasortants that contain 6 segments of Len/17-MDV and the hemagglutinin (HA) and neuraminidase (NA) of contemporary circulating influenza A viruses. The problem with the classical reassortment procedure used to generate LAIVs is that there is limited selection pressure against NA of the Len/17-MDV resulting in 7:1 reassortants with desired HA only, which are not suitable LAIVs. The monoclonal antibodies (mAb) directed against the N2 of Len/17-MDV were generated. 10C4–8E7 mAb inhibits cell-to-cell spread of viruses containing the Len/17-MDV N2, but not viruses with the related N2 from contemporary H3N2 viruses. 10C4–8E7 antibody specifically inhibited the Len/17-MDV replication in vitro and in ovo but didn’t inhibit replication of H3N2 or H1N1pdm09 reassortants. Our data demonstrate that addition of 10C4–8E7 in the classical reassortment improves efficiency of LAIV production.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498426

DOI: 10.1016/j.virol.2018.07.005
PubMed: 30014859
PubMed Central: 6498426

Affiliations:

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines</title>
<author><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carney, Paul" sort="Carney, Paul" uniqKey="Carney P" first="Paul" last="Carney">Paul Carney</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Battelle, Atlanta, GA 30329, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Battelle, Atlanta, GA 30329</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dugan, Vivien G" sort="Dugan, Vivien G" uniqKey="Dugan V" first="Vivien G." last="Dugan">Vivien G. Dugan</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">30014859</idno>
<idno type="pmc">6498426</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC6498426</idno>
<idno type="RBID">PMC:6498426</idno>
<idno type="doi">10.1016/j.virol.2018.07.005</idno>
<date when="2018">2018</date>
<idno type="wicri:Area/Pmc/Corpus">000792</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000792</idno>
<idno type="wicri:Area/Pmc/Curation">000792</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000792</idno>
<idno type="wicri:Area/Pmc/Checkpoint">000127</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000127</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:30014859</idno>
<idno type="wicri:Area/PubMed/Corpus">000019</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">000019</idno>
<idno type="wicri:Area/PubMed/Curation">000019</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">000019</idno>
<idno type="wicri:Area/PubMed/Checkpoint">000019</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">000019</idno>
<idno type="wicri:Area/Ncbi/Merge">000E98</idno>
<idno type="wicri:Area/Ncbi/Curation">000E98</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000E98</idno>
<idno type="wicri:doubleKey">0042-6822:2018:Shcherbik S:monoclonal:antibody:against</idno>
<idno type="wicri:Area/Main/Merge">000176</idno>
<idno type="wicri:Area/Main/Curation">000176</idno>
<idno type="wicri:Area/Main/Exploration">000176</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines</title>
<author><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Carney, Paul" sort="Carney, Paul" uniqKey="Carney P" first="Paul" last="Carney">Paul Carney</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name>
<affiliation wicri:level="2"><nlm:aff id="A2">Battelle, Atlanta, GA 30329, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Battelle, Atlanta, GA 30329</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dugan, Vivien G" sort="Dugan, Vivien G" uniqKey="Dugan V" first="Vivien G." last="Dugan">Vivien G. Dugan</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name>
<affiliation wicri:level="2"><nlm:aff id="A1">Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333, United States</nlm:aff>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Influenza Division, National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, 1600 Clifton Road, Atlanta, GA 30333</wicri:regionArea>
<placeName><region type="state">Géorgie (États-Unis)</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Virology</title>
<idno type="ISSN">0042-6822</idno>
<idno type="eISSN">1096-0341</idno>
<imprint><date when="2018">2018</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Antibodies, Monoclonal (immunology)</term>
<term>Antibodies, Monoclonal (isolation & purification)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibodies, Viral (isolation & purification)</term>
<term>Cell Line</term>
<term>Cold Temperature</term>
<term>Humans</term>
<term>Influenza A Virus, H2N2 Subtype (growth & development)</term>
<term>Influenza A Virus, H2N2 Subtype (immunology)</term>
<term>Influenza A Virus, H2N2 Subtype (isolation & purification)</term>
<term>Influenza Vaccines (immunology)</term>
<term>Influenza Vaccines (isolation & purification)</term>
<term>Neuraminidase (immunology)</term>
<term>Reassortant Viruses (growth & development)</term>
<term>Reassortant Viruses (immunology)</term>
<term>Reassortant Viruses (isolation & purification)</term>
<term>Technology, Pharmaceutical (methods)</term>
<term>Vaccines, Attenuated (immunology)</term>
<term>Vaccines, Attenuated (isolation & purification)</term>
<term>Viral Proteins (immunology)</term>
<term>Virus Cultivation (methods)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Anticorps antiviraux (immunologie)</term>
<term>Anticorps antiviraux (isolement et purification)</term>
<term>Anticorps monoclonaux (immunologie)</term>
<term>Anticorps monoclonaux (isolement et purification)</term>
<term>Basse température</term>
<term>Culture virale ()</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéines virales (immunologie)</term>
<term>Sialidase (immunologie)</term>
<term>Sous-type H2N2 du virus de la grippe A (croissance et développement)</term>
<term>Sous-type H2N2 du virus de la grippe A (immunologie)</term>
<term>Sous-type H2N2 du virus de la grippe A (isolement et purification)</term>
<term>Technologie pharmaceutique ()</term>
<term>Vaccins antigrippaux (immunologie)</term>
<term>Vaccins antigrippaux (isolement et purification)</term>
<term>Vaccins atténués (immunologie)</term>
<term>Vaccins atténués (isolement et purification)</term>
<term>Virus recombinants (croissance et développement)</term>
<term>Virus recombinants (immunologie)</term>
<term>Virus recombinants (isolement et purification)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral</term>
<term>Influenza Vaccines</term>
<term>Neuraminidase</term>
<term>Vaccines, Attenuated</term>
<term>Viral Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antibodies, Monoclonal</term>
<term>Antibodies, Viral</term>
<term>Influenza Vaccines</term>
<term>Vaccines, Attenuated</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Sous-type H2N2 du virus de la grippe A</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Protéines virales</term>
<term>Sialidase</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
<term>Vaccins atténués</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A Virus, H2N2 Subtype</term>
<term>Reassortant Viruses</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Anticorps antiviraux</term>
<term>Anticorps monoclonaux</term>
<term>Sous-type H2N2 du virus de la grippe A</term>
<term>Vaccins antigrippaux</term>
<term>Vaccins atténués</term>
<term>Virus recombinants</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Technology, Pharmaceutical</term>
<term>Virus Cultivation</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cold Temperature</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Basse température</term>
<term>Culture virale</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Technologie pharmaceutique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p id="P1">Cold adapted influenza virus A/Leningrad/134/17/57 (H2N2) is a reliable master donor virus (Len/17-MDV) for preparing live attenuated influenza vaccines (LAIV). LAIVs are 6:2 reasortants that contain 6 segments of Len/17-MDV and the hemagglutinin (HA) and neuraminidase (NA) of contemporary circulating influenza A viruses. The problem with the classical reassortment procedure used to generate LAIVs is that there is limited selection pressure against NA of the Len/17-MDV resulting in 7:1 reassortants with desired HA only, which are not suitable LAIVs. The monoclonal antibodies (mAb) directed against the N2 of Len/17-MDV were generated. 10C4–8E7 mAb inhibits cell-to-cell spread of viruses containing the Len/17-MDV N2, but not viruses with the related N2 from contemporary H3N2 viruses. 10C4–8E7 antibody specifically inhibited the Len/17-MDV replication <italic>in vitro</italic>
 and <italic>in ovo</italic>
 but didn’t inhibit replication of H3N2 or H1N1pdm09 reassortants. Our data demonstrate that addition of 10C4–8E7 in the classical reassortment improves efficiency of LAIV production.</p>
</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
</country>
<region><li>Géorgie (États-Unis)</li>
</region>
</list>
<tree><country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Shcherbik, Svetlana" sort="Shcherbik, Svetlana" uniqKey="Shcherbik S" first="Svetlana" last="Shcherbik">Svetlana Shcherbik</name>
</region>
<name sortKey="Bousse, Tatiana" sort="Bousse, Tatiana" uniqKey="Bousse T" first="Tatiana" last="Bousse">Tatiana Bousse</name>
<name sortKey="Carney, Paul" sort="Carney, Paul" uniqKey="Carney P" first="Paul" last="Carney">Paul Carney</name>
<name sortKey="Dugan, Vivien G" sort="Dugan, Vivien G" uniqKey="Dugan V" first="Vivien G." last="Dugan">Vivien G. Dugan</name>
<name sortKey="Pearce, Nicholas" sort="Pearce, Nicholas" uniqKey="Pearce N" first="Nicholas" last="Pearce">Nicholas Pearce</name>
<name sortKey="Stevens, James" sort="Stevens, James" uniqKey="Stevens J" first="James" last="Stevens">James Stevens</name>
<name sortKey="Wentworth, David E" sort="Wentworth, David E" uniqKey="Wentworth D" first="David E." last="Wentworth">David E. Wentworth</name>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/H2N2V1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000176 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000176 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    H2N2V1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     PMC:6498426
   |texte=   Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:30014859" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a H2N2V1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 14 19:59:40 2020. Site generation: Thu Mar 25 15:38:26 2021

Serveur d'exploration H2N2

Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines

Monoclonal antibody against N2 neuraminidase of cold adapted A/Leningrad/134/17/57 (H2N2) enables efficient generation of live attenuated influenza vaccines

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki

	Serveur d'exploration H2N2
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.